Association-Dissociation Properties of Sodium Borohydride-reduced Phosphorylase b
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چکیده
منابع مشابه
Physical-chemical studies on the pyridoxal phosphate binding site in sodium borohydride-reduced and native phosphorylase.
NaBH4-reduced phosphorylase b is a catalytically active species which retains many of the properties of the native enzyme. One property that is strikingly different, however, is the spectral behavior of bound pyridoxal phosphate. At pH 7.0, the pyridoxal phosphate shows essentially no absorption at 333 nm, where pyridoxal phsophate in the native enzyme absorbs; absorption at 333 mn returns upon...
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Phosphorylase 6 kinase, the enzyme which catalyzes the conversion of phosphorylase b to phosphorylase a, has been extensively studied in extracts of skeletal muscle. Krebs, Graves, and Fischer (1) have reported that this enzyme exists in crude muscle extracts in a form that has very low activity at pH 7 and below. Above pH 7 the activity rises sharply with a maximum at pH 8.5. The enzyme with t...
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Recently we reported on the rapid inactivation of tetracycline by use of cupric-morpholine complex (Kaplan, Lannon, and Buckwalter, J. Pharm. Sci., 64, 163, 1965). This degradation procedure is used as part of an assay technique for kanamycin in the presence of tetracycline. We have since found that sodium borohydride (Metal Hydrides Inc., Beverly, Mass.) can substitute for cupric-morpholine co...
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Cozymase (diphosphopyridine nucleotide, DPN) has been assayed by a variety of biochemical and chemical methods (1). Among the simplest procedures has been the determination of the light absorption of reduced DPN at 340 rnp after reduction by sodium hydrosulfite. Since special precautions are required to remove completely the excess reducing agent which absorbs in this region, it appeared advant...
متن کاملSurface induced dissociation yields quaternary substructure of refractory noncovalent phosphorylase B and glutamate dehydrogenase complexes.
Ion mobility (IM) and tandem mass spectrometry (MS/MS) coupled with native MS are useful for studying noncovalent protein complexes. Collision induced dissociation (CID) is the most common MS/MS dissociation method. However, some protein complexes, including glycogen phosphorylase B kinase (PHB) and L-glutamate dehydrogenase (GDH) examined in this study, are resistant to dissociation by CID at ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1973
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)43708-3